fluocells slide Search Results


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Fisher Scientific fluocells prepared slide #1
Confocal laser <t>microscope</t> optics with a fiber-coupled TES. Two excitation lasers at 405 and 488 nm were combined using a fiber combiner and focused on the sample plane under the objective (×40 NA0.95). Collimated fluorescence photons were focused on the core (10 μm diameter) at the end of the optical fiber, then introduced into the TES. The sample on the focal plane was scanned to accumulate an imaging data.
Fluocells Prepared Slide #1, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Confocal laser microscope optics with a fiber-coupled TES. Two excitation lasers at 405 and 488 nm were combined using a fiber combiner and focused on the sample plane under the objective (×40 NA0.95). Collimated fluorescence photons were focused on the core (10 μm diameter) at the end of the optical fiber, then introduced into the TES. The sample on the focal plane was scanned to accumulate an imaging data.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Few-Photon Spectral Confocal Microscopy for Cell Imaging Using Superconducting Transition Edge Sensor

doi: 10.3389/fbioe.2021.789709

Figure Lengend Snippet: Confocal laser microscope optics with a fiber-coupled TES. Two excitation lasers at 405 and 488 nm were combined using a fiber combiner and focused on the sample plane under the objective (×40 NA0.95). Collimated fluorescence photons were focused on the core (10 μm diameter) at the end of the optical fiber, then introduced into the TES. The sample on the focal plane was scanned to accumulate an imaging data.

Article Snippet: A prepared microscope slide (FluoCells Prepared Slide #1, Tremo Fisher Scientific, USA) was used as a test sample for cell imaging.

Techniques: Microscopy, Fluorescence, Imaging

Color image obtained by a confocal scanning microscope using a TES. A fixed cell sample stained with MitoTracker (red fluorescence for mitochondria), Alexa 488 (green fluorescence for F-actin), and DAPI (blue fluorescence for nuclei). Scanning data of 200 × 200 pixels (0.4 μm for each pixel step) is depicted as (A) RGB color and as (B) NIR images, which were obtained simultaneously. Irradiation power of excitation lasers is indicated. Exposure time of one pixel was 40 ms, and the total duration was 66 min.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Few-Photon Spectral Confocal Microscopy for Cell Imaging Using Superconducting Transition Edge Sensor

doi: 10.3389/fbioe.2021.789709

Figure Lengend Snippet: Color image obtained by a confocal scanning microscope using a TES. A fixed cell sample stained with MitoTracker (red fluorescence for mitochondria), Alexa 488 (green fluorescence for F-actin), and DAPI (blue fluorescence for nuclei). Scanning data of 200 × 200 pixels (0.4 μm for each pixel step) is depicted as (A) RGB color and as (B) NIR images, which were obtained simultaneously. Irradiation power of excitation lasers is indicated. Exposure time of one pixel was 40 ms, and the total duration was 66 min.

Article Snippet: A prepared microscope slide (FluoCells Prepared Slide #1, Tremo Fisher Scientific, USA) was used as a test sample for cell imaging.

Techniques: Microscopy, Staining, Fluorescence, Irradiation